Biocompatibilidade da membrana fibrosa da casca de ovo da gallinea galli gallus domestica: estudo experimental em rato.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Biocompatibility of a membrane is the main requisite for its use in guided tissue regeneration technique (RTG) or guided bone regeneration (ROG). On this research, the response of muscle and connective tissues of Rattus norvegicus albinos to the fibrous membrane extracted from the shells egg of Gallinea galli galus domestica, has been evaluated. The membrane has been removed manually in sterile conditions, washed in a saline solution pH 7.4 and processed into four different types. Type a, after drying, has been packed into sterilized sachets, type b has been inserted into sterilized packages and sterilized by the autoclave, type c has been immersed in a glutaraldehyde solution on 0,5% for fifteen days and then washed, dried and inserted into sterelized sachets and sterelized by the autoclave. Type d has received the same procedures as the type c, except for the sterilization by the autoclave. The evaluation has been produced from histology, immunohistochemical for interferon gamma (IFN-γ) and from enzyme-linked immunosorbent assay for Interleukin 10 (IL-10). The experiment has been done in a 125 rats, divided into group 1, 2 , 3 , 4 ,5 and 6, evaluated for 24 hours, during 1, 2, 3, 8, 10 and 12 weeks. Group 1 has been set to be the non-manipulated rats. In group 2 there has been implantation of membrane type a into their cephalic dorsal area. The rats of group 3 have received on their cephalic dorsal area the membrane b and the cephalic tail area has been manipulated, but with no membrane implants. The animals of group 3 have also been used for the experiments on muscle tissue, which has been implanted the membrane type b into their right back-leg, having the left one as a sham. The membranes c and d have been implanted into the dorsal cephalic and tail area of groups 4 and 5, respectively. In group 6, composed of five rats, there has been implanted into dorsal cephalic and tail area, respectively, the membranes c and d. They have been used to obtain wound fluid. Histological results of membrane type a have shown in three weeks a Sewell gradation classified from light to moderate and there have been many fibroblasts, lymphocyte and plasma cell free. By the end of the twelfth week membrane b has shown a Sewell gradation classified as light, with the presence of a few fibroblasts, an intense network of extracell fibrous matrix, absence of infiltrated inflammatory lymphocyte, but populated by macrophages. The membranes c and d, within eight weeks, have shown a light Sewell gradation, with absence of lymphocyte, plasma cell, macrophage and presence of an extracell fibrous matrix. The immunohistochemical results for the IFN-γ set in connective cells adjoining the membrane type c has shown an absent grade (0 to 0,96%). In group 6, there has been found the presence of IL-10 in the wound fluid. It has been concluded that the membranes type a, b, c and d are biocompatible, but the membranes type c and d have shown a complete biocompatibility, that can be used for the RTG or ROG technique. The presence of IL-10 in the experimental group of one week might have been produced by the macrophages, but the values have not shown to be statistically significant between the membranes type c and d, using the ANOVA test p=0,15. The average diameter of inflammatory reaction in the experimental groups which have used the membranes type c and d, within eight weeks, have not shown statistically significant results under t test, p= 0,17.

ASSUNTO(S)

membrana fibrosa da casca de ovo imunoistoquímica il-10 immunohistochemical histology ifn-γ biocompatibility tecido conjuntivo imunologia eggshells fibrous membrane histologia biocompatibilidade connective tissue tissue fluid fluido de tecido ifn-γ il-10

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