Biochemical, biophysical, and biological properties of densonucleosis virus (parvovirus). III. common sequences of structural proteins.
AUTOR(ES)
Tijssen, P
RESUMO
Densonucleosis virus cannot code for its four structural proteins if each of them has a unique sequence. The objective of the present investigation, therefore, was to establish whether: (i) the viral genome contains overlapping genes; (ii) the virus incorporates host proteins; or (iii) one of the structural proteins is a dimer. Two independent methods were employed for this purpose. First, the viral proteins, solubilized in sodium dodecyl sulfate, were purified after dansylation and were analyzed by peptide mapping, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Second, an enzyme-linked immunosorbent assay was developed for a comparative analysis of the viral proteins solubilized by sodium dodecyl sulfate. It was demonstrated with both techniques that densonucleosis virus has four unique structural proteins, all with extensive sequence homologies. Moreover, all structural proteins contained intraprotein, but no interprotein, disulfide linkages. These results indicated similarities between densonucleosis virus and representatives of the two other genera of the Parvoviridae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170977Documentos Relacionados
- Biochemical, biophysical, and biological properties of densonucleosis virus. I. Structural proteins.
- Biochemical, biophysical, and biological properties of densonucleosis virus (paravovirus). II. Two types of infectious virions.
- Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides.
- Characterization and classification of virus particles associated with hepatitis A. III. Structural proteins.
- Densonucleosis Virus Structural Proteins