Beta-galactosidase and lactose fermentation in the identification of enterobacteria including salmonellae

AUTOR(ES)

Lapage, S. P.

RESUMO

One hundred and fourteen strains of non-lactose fermenters and 127 lactose fermenters on MacConkey's agar have been compared in the 5% and 1% lactose tests and in β-galactosidase production, using ortho-nitro-phenyl-β-D-galactopyranoside (O.N.P.G.) as a test substance. The superiority of the O.N.P.G. test in the number of positive results and its rapidity is shown. In general, late or non-lactose fermenting strains of genera, usually lactose-positive, yield a rapidly positive O.N.P.G. reaction. Forty-one wild strains of Salmonella, Proteus, Providencia, and Pseudomonas aeruginosa were found negative in all three tests.

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