Basis of Pyruvate Inhibition in Thiobacillus thiooxidans1

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RESUMO

Addition of 10−3m pyruvic acid to cultures of Thiobacillus thiooxidans, at pH 2.3, results in its rapid intracellular accumulation and in the cessation of sulfur oxidation, CO2 fixation, and oxygen consumption; at pH 7.0, pyruvate neither inhibits oxygen uptake nor accumulates appreciably intracellularly. Pyruvate does not affect CO2 fixation in cell-free extracts. The data suggest that the cells of T. thiooxidans are passively permeable to pyruvic acid at low pH. Thus entry of pyruvic acid causes accumulation of pyruvate with a concomitant decrease in intracellular pH.

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