Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk.

AUTOR(ES)

Teraguchi, S

RESUMO

When milk-fed mice were orally inoculated with Clostridium ramosum C1, this strain proliferated in the gut and became the dominant component of the fecal microflora. In this experimental model, bovine lactoferrin (bLF) administered with milk suppressed the proliferation of this strain in vivo and decreased the numbers of C. ramosum and other bacteria in the feces. This bacteriostatic effect of bLF was dependent on the concentration of bLF, the duration of feeding, and the administered dose of C. ramosum C1. Compared with bovine serum albumin, ovalbumin, bovine whey protein isolate, or bovine casein, only bLF showed this specific activity. A similar effect of bLF was observed after oral inoculation with C. ramosum JCM 1298, C. paraputrificum VPI 6372, or C. perfringens ATCC 13124. A hydrolysate prepared by digestion of bLF with porcine pepsin showed the same inhibitory effect on proliferation of C. ramosum in vivo as occurred with undigested bLF. These results indicate that ingested bLF can exert a bacteriostatic effect against clostridia in the gut even after it has been digested to some extent.

Documentos Relacionados

• Orally administered bovine lactoferrin inhibits bacterial translocation in mice fed bovine milk.
• Effect of orally administered beclomethasone dipropionate on calcium absorption from the gut in normal subjects.
• Effect of orally administered monoclonal antibody on persistent Cryptosporidium parvum infection in scid mice.
• Bacteriostatic effect of human milk and bovine colostrum on Escherichia coli: importance of bicarbonate.
• Effect of orally administered probenecid on the pharmacokinetics of cefoxitin.