Bacteriological assessment and multiplex-PCR test for the detection of meat adulteration of different animal species
AUTOR(ES)
ABUELNAGA, Azza Sayed Mohamed; ABD EL-RAZIK, Khaled Abd El-Hamid; ATA, Nagwa Sayed; HEDIA, Riham Hasaan; ELGABRY, Elgabry Abd-Elalim; SOLIMAN, Mona Mohamed Hassan; MARIE, Hanan Shawky Abdel Wahab
FONTE
Food Sci. Technol
DATA DE PUBLICAÇÃO
2021-06
RESUMO
Abstract Meat samples of various animal species have been obtained from Giza Governorate's butcher shops, common retail markets, veterinary faculties, Zoo and hospitals. Physical, bacteriological, and molecular analysis was carried out on the meat samples. Physical examination showed that typical and untypical meat differed. Bacteriological examination showed the highest TCC was found in horse meat while the least was in goat meat. The total count of S. aureus was the highest in donkey and dog whilst the least in pork meat. As for E.coli, donkey meat was the highest while buffalo and mutton meat were the lowest. The highest total Listeria monocytogenes and Salmonella Species count were in horse, donkey, rat and dog meat but zero in the other species examined. Multiplex-PCR targeting partial-length of cytochrome b (cyt b) gene of mitochondrial DNA (mtDNA) was used for the discovery of adulteration of beef meat with chicken, pork, dog, cat and rat tissue. It showed high specificity in differentiating the six animal species meat. The study points out the importance of taking serious steps to control species meat adulteration that may lead to transmission of severe foodborne diseases and more studies need to be implemented to apply new and easy meat adulteration detection protocols.
Documentos Relacionados
- Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica
- Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
- Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
- Determinação de sorotipos capsulares de Streptococcus pneumoniae por Multiplex-PCR sequencial
- An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders