Bacterial Toxin-Antitoxin Gene System as Containment Control in Yeast Cells
AUTOR(ES)
Kristoffersen, P.
FONTE
American Society for Microbiology
RESUMO
The potential of a bacterial toxin-antitoxin gene system for use in containment control in eukaryotes was explored. The Escherichia coli relE and relB genes were expressed in the yeast Saccharomyces cerevisiae. Expression of the relE gene was highly toxic to yeast cells. However, expression of the relB gene counteracted the effect of relE to some extent, suggesting that toxin-antitoxin interaction also occurs in S. cerevisiae. Thus, bacterial toxin-antitoxin gene systems also have potential applications in the control of cell proliferation in eukaryotic cells, especially in those industrial fermentation processes in which the escape of genetically modified cells would be considered highly risky.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92497Documentos Relacionados
- The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
- A Novel Family of Escherichia coli Toxin-Antitoxin Gene Pairs
- Killing Effect and Antitoxic Activity of the Leptospira interrogans Toxin-Antitoxin System in Escherichia coli
- Crystallization of Doc and the Phd–Doc toxin–antitoxin complex
- Toxin-Antitoxin Modules May Regulate Synthesis of Macromolecules during Nutritional Stress