Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles.
AUTOR(ES)
Stenström, T A
RESUMO
The adhesion of Salmonella typhimurium to the mineral particles quartz, albite, feldspar, and magnetite was shown to correlate with the hydrophobicity of the cell surface as measured by hydrophobic interaction chromatography. The same effects were also seen for seven other selected test strains, including Streptococcus faecalis, Streptococcus faecium, Escherichia coli, Citrobacter freundii, Shigella sonnei, and Shigella boydii. When the test strain of Salmonella typhimurium, was repeatedly cultivated in Luria broth, thus selecting for different degrees of fimbriation and roughness of the cell surface, varied cell hydrophobicity but constant negative and positive charge values were obtained. High hydrophobicity values always coincided with enhanced adhesion to the mineral particles. The negative charge of the bacterial surface as measured by electrostatic interaction chromatography appeared to play no role in the adhesion event. However, the positive charges on the cell surface contributed to the adhesion process. This was especially evident for cells exhibiting a high degree of hydrophobicity. Alteration of the pH between 4 and 9 did not significantly affect the adhesion process.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=184068Documentos Relacionados
- Hydrophobicity, Adhesion, and Surface-Exposed Proteins of Gliding Bacteria
- Growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse.
- The role of bacterial cell wall hydrophobicity in adhesion.
- Comparison between the adhesion to solid substrata of Streptococcus mitis and that of polystyrene particles.
- Bacterial Adhesion to Soil Contaminants in the Presence of Surfactants