Axonal calcium entry during fast 'sodium' action potentials in rat cerebellar Purkinje neurones.
AUTOR(ES)
Callewaert, G
RESUMO
1. Using laser-scanning confocal microscopy, fast Ca2+ transients were recorded in individual not yet myelinated axons of Purkinje neurones in cerebellar slices from young rats. Axonal Ca2+ transients could be detected during a single action potential and had progressively larger amplitudes when the number of action potentials was increased. 2. Under voltage-clamp conditions, axonal Ca2+ transients were as large as those observed in dendrites and in the cell body. Axonal Ca2+ transients were completely blocked by 100 nM of the neurotoxin omega-agatoxin IVA, indicating that they were caused by Ca2+ entry through P-type voltage-gated Ca2+ channels. 3. In conclusion, our results demonstrate action potential-mediated Ca2+ entry through voltage-gated Ca2+ channels in axons of cerebellar Purkinje neurones. Experimental evidence indicates that the resulting transient Ca2+ accumulations regulate the frequency of action potentials travelling along the axon.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1160771Documentos Relacionados
- Ryanodine receptor-mediated intracellular calcium release in rat cerebellar Purkinje neurones.
- Fast activation and inactivation of inositol trisphosphate-evoked Ca2+ release in rat cerebellar Purkinje neurones.
- Sodium action potentials in the dendrites of cerebellar Purkinje cells.
- Calcium and sodium currents evoked by action potential waveforms in rat sympathetic neurones.
- Spatial profile of dendritic calcium transients evoked by action potentials in rat neocortical pyramidal neurones.
