Avaliação Quantitativa e Qualitativa das Proteínas dos Ovos de Rhipicephalus (Boophilus) microplus e Rhipicephalus (Rhipicephalus) sanguineus (Acari: Ixodidae) Durante a Oviposição e Embriogênse. / Quantitative and Qualitative Avaluation of Eggs Proteins of Rhipicephalus (Boophilus) microplus and Rhipicephalus (Rhipicephalus) sanguineus (Acari: Ixodidae) During Oviposition and Embryogenese.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

To fill some gaps on intrinsic mechanisms of the biology of the oviposition and embryogenesis of R. (B.) microplus and R. (R.) sanguineus, was evaluated the variability protein in eggs per day of posture and incubation. For this, engorged female laid eggs in controlled environment chamber (27 1oC, 80 5% UR, and darkness). As soon as the female initiated the oviposition, egg samples of 50 mg was collected daily, conditioned in eppendorf tube and preserved in freezer - 20C, characterizing the period of posture. Samples of 50 mg was removed from a fresh egg mixture and put in perforated eppendorf tube, kept in environment chamber (27 1oC, 80 5% UR, and darkness). Daily, one tube was transferred to freezer -20C until the first larva hatch. Thus a sequence of different stage of embryogenesis was obtained. The Bradford method was used to measure the protein concentration and subsequently, the electrophoresis profiles was performed in SDS-PAGE. The protein concentrations was correlated with the oviposition and embryogenesis days using the Pearson (r) correlation and for this, the data was transformed in logarithmic value [log (X+1)] after the normality to be discarded (test of Shapiro-Wilk). During oviposition the protein concentrations of the eggs of R. (B.) microplus and R. (R.) sanguineus remained constant until the last days when abrupt increase was observed. In both of species, variation in the concentration of protein was observed during all embryonic period. The number of detectable bands of proteins decreasing during oviposition and embryogenesis days, but in the last days a new band was found. It can inferred that the proteins variation in the eggs of R. (B.) microplus and R. (R.) sanguineus is correlated with the days oviposition and incubation. The ticks R. (B.) microplus and R. (R.) sanguineus have a different oviposition profile proteins model that can be use as phenetic feature. As well, a different way of degradation of protein for each species was characterized.

ASSUNTO(S)

carrapato tick posture eletroforese. postura medicina veterinaria electrophoresis

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