Avaliação neurológica e histológica de lesão compressiva da medula espinhal de ratos wistar, tratados com células-tronco mesenquimais / Mesenchymal stem cells therapy for spinal cord injury in Wistar rats: neurological recovery and histological changes

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

14/03/2011

RESUMO

Effective treatment for spinal cord injury remains subject of several studies. Cell therapy is considered an option promising for lesions of the central nervous system, particularly stem cells after experimental animal models showed the transplantation of bone marrow-derived cells results in amelioration of the functional deficit in various neurological diseases. This work evaluated the effect of an intravenous injection of mesenchymal stem cells (MSCs) in different intervals after compressive spinal cord injury compared to treatment with methylprednisolone (MP) and no treatment. MSCs from rat bone marrow were cultivated and expanded in vitro until transplantation. Spinal cord injury was performed with 2-Fr Fogarty catheter after T9-T10 laminectomy. Animals were randomly divided into five experimental groups with 10 animals each and subjected to different treatments: group (CG), which received only PBS; MP group (GM), who received 30 mg/kg SSMP three hours after injury, group 3 hours (GCT), which received administration of MSCs three hours after induction of injury, group 3 days (G3D), received the MSC after three days of injury; and group 7 days (G7D), received MSC seven days after spinal cord injury. Animals were evaluated weekly using the Basso-Beattie-Bresnahan (BBB) open field locomotor test. 35 days after the lesion was performed histological evaluation. All groups treated with MSC showed better results in the motor recovery. The BBB test revealed no difference between GC and GM. In morphometric analysis, the groups treated with MSC showed minor injury area and a higher percentage of healthy tissue than other groups. No differences were observed between groups in the GM and GC analysis histology. It was found that cell therapy with MSCs contributes positively in clinical recovery and preservation of nervous tissue after compressive spinal cord injury.

ASSUNTO(S)

terapia celular trauma medular recuperação neurológica medicina veterinaria cellular therapy spinal cord trauma neurological recovery

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