Avaliação dos efeitos genotóxico e citotóxico do 153Sm-EDTMP em linfócitos periféricos de pacientes com metástase óssea / Evaluation of genotoxic and cytotoxic effects of 153Sm-EDTMP in peripheral blood lymphocytes of bone metastasis patients

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

In this study the cellular damage in peripheral lymphocytes after exposure to 153Sm-EDTMP (Samarium-153 ethylenediaminetetrametylenephosphonate) was determined using the technique of micronuclei analysis and differential coloration. 153Sm- EDTMP is a radiopharmaceutical used for pain relief in patients with bone metastases. The analysis of the frequency of micronuclei in patient blood samples obtained one hour after endovenous administration of radiopharmaceutical (41 MBq/kg) showed no statistical difference in relation to basal values in binucleated cells. However the analysis of damage distribution in mononucleated cells, showed that the patients without previous radiotherapic treatment presented a significant increase in the frequency of cells with one micronucleus and in those who had taken previous radiotherapic treatment, in cells with two or more micronuclei. The in vitro experiments conducted with the exposition of total blood to three radiation concentrations of 153Sm-EDTMP (0.370, 0.555 and 1.110 MBq/mL) during one hour showed an increase in the frequency of micronuclei and necrotic and apoptotic cells with increasing radiation dose. Dose-response curves for healthy donors and patients with bone metastasis without previous radiotherapic treatment were constructed. The comparison of the curves showed that patients presented higher radiosensitivity, either micronuclei or dead cell (necrotic or apoptotic) percentages, than healthy donors.

ASSUNTO(S)

amarium 153 biological radiation effects blood cells cell killing cell nuclei células de sangue efeitos da radiação biológica in vitro in vivo linfócitos lymphocytes metastases neoplasms pain

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