Avaliação dos efeitos citotóxicos e antiproliferativos da alfa-tomatina em células tumorais não-pequenas de pulmão

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

30/03/2011

RESUMO

α-Tomatine is a glycoalkaloid present in different parts of the tomato plant (Lycopersicon esculentum), found in great amounts in the unripen fruits. Many different biological properties have been described for this compound such as antimutagenic, antinflamatory, imunnorregulator, fungicide and bactericide properties. Lung cancer is a deathly disease; its mortality is related to the after-diagnosis procedures such as surgical resection and non-responsive chemotherapy. This work aims evaluating α-tomatine effects on inhibition of cellular growing, apoptosis-triggering and gene regulation in non-small lung tumor cells. To achieve this goal we will use in vitro NCI-H460 cells. Our results showed that α-tomatine inhibited cell growing successfully at 2µg/mL at the first 24 hours by 25%, inhibiting it completely at 5µg/mL or above. α-Tomatine didn’t show any apoptosis-triggering property or genotoxic effects. α-Tomatine didn’t change CYP1A1, P53 or GST gene expression rates at 0,1 and 2µg/mL. These results suggest that α-tomatine doesn’t trigger apoptosis through the classical pathways already described, and its in vitro inhibitory effect on tumor cells is likely due to its interaction with cell membrane following lisis. Many works have already shown α-tomatine as a potent antitumor compound. Our work contributes to the newest researches and suggests new works aiming to elucidate α-tomatine biochemical pathways.

ASSUNTO(S)

pulmões - câncer células cancerosas - proliferação apoptose - indução células cancerosas - efeito citotóxico tomate - aspectos biológicos lungs cancer cancer cells tomatoes biological aspects

ACESSO AO ARTIGO

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