Avaliação do perfil metabólico da estavudina através do emprego da bioconversão e da modelagem molecular do citocromo P-450 CYP3A4 / Evaluation of the metabolic profile of stavudine through the use of bioconversion and molecular modeling of cytochrome P-450 CYP3A4

AUTOR(ES)

Lênis Medeiros de Freitas

DATA DE PUBLICAÇÃO

2009

RESUMO

Before the approval of an active compound, metabolism studies are necessary to ensure its safety, once active metabolites could be synthesized during human biotransformation. The use of eukaryotic microorganisms for the study of drug metabolism has been widely explored, due to its capability of producing metabolites similar to the mammalians, and in silico studies consist in a fast strategy when compared with traditional metabolism studies. In this context, molecular modeling, using docking of molecules of interest in the active site of enzymes involved in drug metabolism, is a useful tool to evaluate the interactions between drug and receptor, because it could predict favorable orientations that could be biotransformated. In this work, sixteen filamentous fungi strains, obtained from collections and isolated from soil in the central Brazil, were evaluated for their capability of the antiretroviral stavudine biotransformation, also complemented by animal metabolism studies and molecular modeling of the most relevant cytochrome P450 isoform of human metabolism: CYP3A4. From the bioconversion experiments, the fungus Cunninghamella elegans ATCC 26169 was capable of metabolize stavudine, forming mammalian metabolites, producing the thymine derivative. Dynamic molecular studies demonstrated that the most probable reactions for stavudine, catalyzed by CYP3A4, involves hydroxylation of methyl group (position C-7) and the double bond epoxidation of the furanic ring, showing the importance of some residues of the active site in this process, like Arg212

ASSUNTO(S)

biotransformation, eukaryotic microorganisms, molecular modeling, stavudine biotransformação, microrganismos eucarióticos, modelagem molecular, estavudina farmacotecnia

Documentos Relacionados

• Avaliação do perfil metabólico da estavudina através do emprego da bioconversão e da modelagem molecular do citocromo P-450 CYP3A4
• Chloramphenicol Is a Potent Inhibitor of Cytochrome P450 Isoforms CYP2C19 and CYP3A4 in Human Liver Microsomes
• Avaliação da presença do polimorfismo Msp1 da CYP1A1 do citocromo P-450 em mulheres assintomáticas portadoras de cisto mamário.
• Cloning and expression of a member of a new cytochrome P-450 family: cytochrome P-450lin (CYP111) from Pseudomonas incognita.
• Tetranucleotide repeat polymorphism at the human aromatase cytochrome P-450 gene (CYP19)