Avaliação do efeito do perfluoroctano e do perfluorohexiloctano sobre cultura de celulas Vero

AUTOR(ES)

Paulo Estacia

DATA DE PUBLICAÇÃO

2003

RESUMO

Perfluorocarbon liquids (PFCLs) are used in eye surgeries, not only as an intraoperative tool, but also as intraocular tamponading agents. However, some authors state that perfluoroctane (PFOC), a type of PFCL, may cause inflammatory reactions and damage the retina. In an attempt to avoid these effects, semifluorinated alkanes (SFA) were developed as long-term vitreous replacements. Therefore, we comparatively assessed the potential toxic effect ofPFOC and perfluorohexyloctane (F6Hs), a type ofSFA, under controlled cell culture conditions. Vero cells were used for this purpose, as internationally recommended for cytotoxicity tests and studies with biomaterials. We analyzed indirect cytotoxicity, where the cells only come into contact with soluble elements that can be eliminated by PFOC and F_s, in addition to direct toxicity (contact toxicity) of PFOC or F6Hs to the cells. Indirect toxicity was assessed quantitatively by way of absorbance, indicating cell viability. In its turn, direct toxicity was evaluated by cytochemical, immunocytochemical and morphological methods. Cells embedded in a treatment-free culture medium were used as control- a positive control for toxicity (silicone bands), with an undeniably toxic effect on cells, and a weight control (glass slides) that produced a mechanical compression similar to the amount of PFOC or F 6Hs used in the experiment. Both direct and indirect toxicity were fIrst assessed separately and, later on, collectively and comparatively. Our results show that PFOC does not produce indirect toxicity, but it causes some toxic effects mediated by direct contact; and that F6H8 did not present direct toxicity. Differently from PFOC, cellular alterations caused by F6H8 were similar to those produced by the weight control and different from the toxicity controI. Therefore, our conc1usion is that F6H8 has a compressive rather than a direct toxic effect. These fIndings were corroborated by the integrated and comparative study, thus strengthening our conc1usions. The joint analysis of our data revealed that F6Hs has better properties as long-term vitreous replacement than PFOC, since it showed a lower specifIc weight and no toxic effect, which resulted in a lighter compressive action on the cultured cells

ASSUNTO(S)

retina cultura celular

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