AVALIAÇÃO DE PARÂMETROS BIOQUÍMICOS NUTRICIONAIS E DO ESTRESSE OXIDATIVO EM RATOS TRATADOS COM EXTRATO OLEOSO DE BIXINA (P.A. LIPO 8%)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The annato seeds extract used as a food colour has also been described by the scientific community by its hypocholesterolemic effect. Thus this work aimed at assessing in rats the effect of the administration of an oil extract containing 8% bixin on the lipid profile, antioxidant defenses and markers of hepatic and renal functions. Three experiments were conducted: in the first one male rats received the AOAC diet, in the second one female rats were fed the AOAC diet and in the third one female rats were given the AIN-93 diet. Each experiment lasted for eight weeks and in each one 32 Fisher rats were used which were divided into four groups: control (C) that received the standard diet (AOAC or AIN-93), H that received a hypercholesterolemic diet, CBO was given the standard diet supplemented with the oil extract of bixin and HBO that received the hypercholesterolemic diet supplemented with the extract. After the experimental period samples of blood and hepatic tissue were collected and biochemical determinations were performed. Our results for the first experiment indicated that the association of bixin with the hypercholesterolemic diet reduced the concentrations of total and non-HDL cholesterol as well as reduced the atherogenic index, the amount of hepatic and fecal fat and the activities of ALT and AST in the animals of the HBO group. The association of bixin with the control diet raised the concentration of HDL-cholesterol and reduced the AST activity in the CBO animals. Bixin exerted a hypotriglyceridemic effect, reduced the concentration of urea and raised that of total and free sulphydryl groups besides increasing the ALP activity. The results of the second experiment indicated that the association of bixin with the hypercholesterolemic diet reduced the concentrations of non-HDL cholesterol, the atherogenic index, the activities of ALT and ALP, the amount of hepatic fat, as well as the concentrations of cholesterol and hepatic and fecal triacylglicerols, and the concentration of creatinina in the HBO animals. This association increased the serum concentrations of HDL cholesterol and triacylglycerols and the arilesterasic and paraoxonase activities of PON. The association of bixin with the control diet increased the ALT activity and the concentration of urea and reduced the concentration of creatinina and the arilesterasic and paraoxonase activities of PON in the CBO rats. exerted a hypocholesterolemic effect and raised the concentration of free sulphydryl groups. In the third experiment the results indicated that the association of bixin with the hypercholesterolemic diet increased the concentrations of total and non-HDL cholesterol, raised the atherogenic index, the amount of fecal fat, the fecal triacylglicerols and the ALP activity, as well reduced the paraoxonase activity of PON of the HBO animals. The association of bixin with the control diet raised the concentrations of HDL, of hepatic triacylglicerols, of free sulphydryl groups and the arilesterasic activity of PON and decreased the serum concentration of creatinina of the CBO animals. Bixin exerted a hypotriglyceridemic effect and raised concentration of free and protein-bound sulphydryls groups. In the proposed conditions and models we conclude that the treatment with bixin exerted a hypocholesterolemic effect in experiments 1 and 2 where the AOAC diet was offered. On the other hand we observed that the hypocholesterolemic effect induced by this treatment was not observed with the AIN-93 diet what suggests that the variations related to the composition of the diets might have influenced the action of bixin in the lipid metabolism. The experiments indicated also satisfactory results as for the antioxidant properties of bixin and that the alterations of markers of hepatic and renal functions may be related to the particular variations of each experiment.

ASSUNTO(S)

bioquimica 1. colesterol - teses. 2. hipercolesterolemia - teses. 3. estresse oxidativo - teses. i. universidade federal de ouro preto. ii. título.

ACESSO AO ARTIGO

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