Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo / Assesment of Motility Chacarteristics (CASA), morphology; plasmatic membrane funcionality (HOST) in flow citometry sex sorted bovine sperm

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Sex selecting has a huge impact in dairy and beef production. The only way commercially viable to obtain sex selecting nowadays, is a flow citometry sex sorted sperm technique. Currently sex sorted sperm have been used regularly in Artificial Insemination programs. Meanwhile, there are limitations about non return rate in farm conditions, superficially explained as cellular loss integrity. The objective of this experiment was assessing sex sorted sperm effects and time to wait 0, 3 and 6 hours before sorting in motility parameters by computer assisted sperm analysis (CASA), plasmatic membrane functionality by hiposmotic swelling test (HOST), sperm morphology by differential interference contrast microcopy (DIC) and between taurine subspecies (Bos taurus taurus) zebuine subspecies (Bos taurus indicus). Sex sorted sperm showed best results in the most motility parameters compared with conventional sperm, suggesting a flow citometer selection. The time to wait of the sperm (untis 6 hours) to keep sorting, altered a linearity (LIN). Plasmatic membrane functionality was affected when sperm runs through flow citometer.. Sex sorted induces increase of minor defects in sperm, probably by tail membrane plasmatic alterations. Zebuin sperm are more sensitive for tail plasmatic membrane alterations compared with taurine sperm. Finally, there is strong indication that bovine sperm that runs through flow citometer sign in hiperactivation state.

ASSUNTO(S)

bovine bovinos computer assisted motility analysis hyposmotic swelling test patologia espermática sêmen sexado sexed semen sistema computadorizado de avaliação da motilidade sperm pathology teste hiposmotico

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