Avaliação da reparação tecidual de excisões realizadas em dorso de ratos submetidas à terapia foto-dinâmica com utilização de corante azul de metileno / Wound healing evaluation of excisions performed on the back of rats and submitted to photodynamic therapy mediated by methylene blue dye

AUTOR(ES)

Felipe Fornias Sperandio

DATA DE PUBLICAÇÃO

2009

RESUMO

The photodynamic therapy involves delivering visible light of the appropriate wavelength into a tissue or microorganism previously exposed to a photo-sensitive dye. Its use is widely spread between neoplastic and infeccious diseases. Nevertheless, its effects upon wound healing has not yet been completely verified and the few studies concerning this subject present whether good or bad results. In addition, studies that involve low intensity laser therapy or photodynamic therapy on wound healing concern mostly on the organization and quantification of the extracellular matrix components. Studying the epithelium, on the other hand, by the keratinocyte expressed proteins is equally important, once the wound healing depends also of the organization and proliferation of the epithelial cells. This study evaluated the wound healing of excisions performed on the back of rats submitted to low intensity laser therapy or photodynamic therapy mediated by methylene blue dye. Morphological and histo-morphological analysis of the wounds in pre-determined periods were performed, as well as immunohistochemistry of citokeratins 10 and 14 and p63. The results showed that the wound healing was enhanced by the low intensity laser therapy, which was confirmed by the morphological and histomorphological analysis. The wound closure was previously seen for the laser group. The citokeratin 10 expression on the epithelial tongue of the wounds that belong to the laser group preceded the expression in the other groups, which indicated an accelerated maturation of this epithelium. The photodynamic therapy group did not present accelerated wound healing but did not present any delay as well. This suggests that the wound healing found for the laser group differs from that found for the photodynamic therapy group. Moreover, the lack of delay presented by the photodynamic therapy suggests a safe therapy with advantages regarding disinfection that should be considered in specific situations.

ASSUNTO(S)

citokeratin 14 low intensity laser therapy wound healing citoqueratina 14 p63 citokeratin 10 terapia fotodinâmica laser em baixa intensidade citoqueratina 10 photodynamic therapy reparação tecidual p63

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