Avaliação da osteointegração do copolímero PHBV obtido de Chromobacterium violaceum, em falha óssea produzida no fêmur de ratos wistar

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Biopolymers have attracted great interest as alternative materials for wound and lost tissues treatments. The objective of this work is the study of the interaction between biological tissues and scaffolds produced from polyhydroxialkanoates (PHAs). PHAs are biodegradable and bioresorbable polymers. Scaffolds of PHA were produced in a bioreactor from poly(3-hydroxybutirate-co-3-hydroxyvalerate) (PHBV) incorporated with violacein. The material was pressed and partially sintered to produce scaffolds with 3 mm of diameter and 3 mm of height. The scaffolds were implanted in femur of Wistar rats according to a protocol developed in this work. X-ray radiographs were taken after surgery. The materials were extracted and analyzed after 30 and 60 days of surgery. Microstructural characterization and chemical analysis to determine the inorganic constituents and the Ca:P ratio of the implanted biopolymers were evaluated by scanning electron microscopy (SEM) coupled with X-ray dispersive energy spectroscopy (EDS). Histological evaluation revealed that no inflammatory reaction occurred and new bone tissue was formed in the implant. The results indicated that PHBVs with violacein are potential candidates for application in regenerative medicine, especially in bone tissue engineering.

ASSUNTO(S)

biopolimeros materiais biocompatíveis polihidroxialcanoatos engenharia quimica engenharia quimica violaceína biocompatible materials

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