Avaliação da expressão das proteínas p53 e pRb em carcinoma escamocelular e papilomas orais pelo método imuno-histoquímico. / Evaluation of p53 and pRb proteins from oral squamous carcinoma and papillomas by immunohistochemical methods.

AUTOR(ES)

Leila Brito de Queiroz

DATA DE PUBLICAÇÃO

2006

RESUMO

The cell cycle progression is regulated by proteins like the cyclin dependent kinases, cyclin depedent kinases inhibitors and tumour suppressor proteins like, p53 and pRb. Alterations of the tumour suppression proteins can be responsible for the development of malignant processes. The HPV, a epitheliotropic virus, is associated with cervical cancer and encodes two oncoproteínas E6 and E7, that induce cellular transformation biding to the p53 and pRb respectively. In this study we proposed to evaluate the immunohistochemistry expression of the p53 and pRb proteins from paraffin embedded tissues of oral carcinoma (n=31), oral carcinoma (n=19) and normal tissue (n=6) and associate the results with the detection of HPV DNA by PCR. The immunoexpression of the nuclear proteins eas scored according the percentation of positive cells in score 0, 1 and 2 (<10%, , between 10% and 50% and more than 50% of immunoexpression, respectively). In the cases of carcinoma the p53 and pRb were both expressed in 63,3%. In the samples of papillomas, 5,3% and 26,3% were positive for the immunoexpression of p53 and pRb, respectively. In normal tissue 50% was positive for p53 and 16,7% for pRb. Was found significant correlation between the detection of p53 and pRb and the diagnosis of benign or malignant lesions (p=0,000). It was also significant the correlation between the ranks for detection of pRb in carcinoma and normal tissues. The protein overexpression (score 2) was associated with the development of malignant neiplasia. In this study was not possible to associate the immunoexpression of the proteins with the detection of HPV.

ASSUNTO(S)

carcinoma escamocelular oral p53 prb imunologia p53 hpv pcr hpv imuno-histoquimica oral cell squamous carcinoma immunohistochemistry pcr prb

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