Avaliação da eficácia do tratamento específico com Benznidazol na progressão da doença de Chagas Experimental e a correlação entre a eficácia do tratamento nos modelos canino e murino

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

In this present work, it was evaluated the benefit of precocious Benznidazole (Bz) treatment of experimental Chagas disease in reduce cardiac lesions in dogs and mice previously inoculated with sensitive (Be-78), partially sensitive (Y, ABC and AAS) and resistant (VL-10) strains of Trypanosoma cruzi. In addition, it was also evaluated the interference of this specific Bz treatment on the standard of mice (IgG, IgG1 and IgG2) and dogs (IgG1, IgG2a and IgG2b) antibodies in the attempt to correlate them with suggestive circulating markers of cure criteria during Chagas disease. Bz treatment was able to prevent inflammation and fibrosis in heart from infected dogs and mice that reached parasitological cure, except in those treated dogs inoculated with Y strain of T. cruzi that presented cardiac fibrosis. Among Bz treated and not cured dogs, it was observed: (i) no reduction on the inflammation and fibrosis in those animals inoculated with ABC and VL-10 strains; (ii) reduction on cardiac lesions in those inoculated with AAS strain. On the other hand, in murine model, the specific treatment was not able to reduce the inflammation intensity in those mice treated and not cured previously infected with ABC, AAS and VL-10 strains. In this case, Bz effectively prevented cardiac fibrosis in all mice inoculated with VL-10 strain. Interestingly, serological investigation demonstrated a close relation between high levels of IgG1 and intensity of cardiac inflammation in all not-treated animals (dogs and mice) when they were infected with different T. cruzi strains. The specific Bz treatment induced a significant reduction in levels all immunoglobulins investigated. However, a negative serological data was just observed in those treated and cured animals while similar results were observed between immunoglobulins from those treated and not-cured and infected control animals. Our data suggest that specific Benznidazole treatment prevents cardiac lesions in animals that reached parasitological cure and circulating immunoglobulins appears as promising markers of cure criteria during experimental Chagas disease.

ASSUNTO(S)

imunologia 1. tripanossoma cruzi - teses. 2. quimioterapia - teses. 3. chagas, doença de - teses. 4. cão - teses. i. universidade federal de ouro preto. ii. título.

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