AVALIAÇÃO DA ATIVIDADE FARMACOLÓGICA DE Cissus verticillata Nicolson &C. E. Jarvis subsp. verticillata COMO ANTIOXIDANTE, ANTIFÚNGICO, HIPOGLICEMIANTE E CICATRIZANTE / Assessment of pharmacological activity of Cissus verticillata Nicolson &C. E. Jarvis subsp. verticillata as antioxidant, antifungal, hypoglycaemic and healing

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

BRAGA, Tatiane Vieira. Universidade Federal de Ouro Preto, july,2008. Assessment of pharmacological activity of Cissus verticillata Nicolson &C. E. Jarvis subsp. verticillata as antioxidant, antifungal, hypoglycaemic and healing. Adviser: Tanus Jorge Nagem. Co-advisor: Tânia Toledo de Oliveira. The species Cissus verticillata (L.) Nicolson &C. E. Jarvis subsp. verticillata is popularly known as vegetable insulin and is used in diabetes treatment. In this work it was evaluated, at first, the process of crop and drying of the vegetal sample and the commercial samples quality of vegetal insulin. The vegetal material was collected in Ouro Preto, Minas Gearais, and the excicata was stored in the José Badini Herbarium of UFOP (OUPR 20570). The leaves of the C. verticillta verticillata have an amount of water by 85,52%. The drying process in the oven with forced ventilation, 45C of temperature, was efficent, leading to leaves with 11.47% of humidity and 17.99% of total ashes. In the commercial samples of vegetal Insulin high values of mesophilic aerobic load were found. Afterwards, it was evaluated the chromatographic profile of C. verticillata verticillata which confirmed the presence of rutin in the fraction ethyl acetate of the leaves. The antioxidant activity was also evaluated by determination of the total phenolics compound and the DPPHs methodology. The quantification of total phenolics compounds in EHA, FD and FA was, respectively, 381.0, 52.0 and 2574.0 mg/mL in equivalent of tanic acid. The antioxidant activity by the methodology of DPPH was of 11.68, 20.24 and 70.06 in EHD, FD and FA, respectively. It was determined the antifungal activity aiming at new antifungal compounds of vegetal origin that are able to auxiliary the therapeutics. The FD had CIM of 125 ug/mL for C. krusei, and the FC had CIM of 125 ug/mL for C. xxiv tropicallis. In the evaluation of the hipoglycaemic activity the dose of 4.5 mg/Kg reduced the glycemia by 35% and the dose of 13.5 mg/Kg was more efficent, reducing the glycemia by 60% in 28 days. None of the assessed doses modified the plasmatic levels of insulin. The 13.5 mg/Kg doses reduced the triglycerides levels by 70%. The extract of C.verticillata verticillata helped the process of healing of skin wounds in rabbits.

ASSUNTO(S)

1. antioxidantes - teses. 2. cissus verticillata - teses. 3. fitoterápicos - teses. i. universidade federal de ouro preto. ii. título. farmacia

ACESSO AO ARTIGO

Documentos Relacionados