AVALIAÇÃO CINÉTICA DO SÊMEN DE PIRAPITINGA (Piaractus brachypomus) CONGELADO EM MEIO À BASE DE ÁGUA DE COCO EM PÓ (ACP-104) OU RINGER EM TRÊS MEIOS DE ATIVAÇÃO

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The pirapitinga, Piaractus brachypomus (CUVIER, 1818) is a Characidade freshwater fish that inhabits the Amazon and Orinoco River basins. The aim of this study was determinate spermatic motility of cryopreserved sperm of pirapitinga in three different activation solutions. Sperm samples were diluted in four freezing media prepared by a combination of two extenders ACP-104, coconut water pounder and RINGER modificated and two cryoprotectant agents (DMSO and methylglycol). Data shows NaCl and NaHCO3 results were similar to each other and significantly superior to distilled water. The ACP-104 treatment associated with the cryoprotectant DMSO showed activation of sperm motility after thawing significantly higher compared to other treatments. In conclusion, therefore, the coconut milk powder modified to keep fish sperm motility feasible and proved to be a good diluent for sperm cryopreservation in this species.

ASSUNTO(S)

piaractus brachypomus sêmen piaractus brachypomus cryopreservation peixe criopreservação fish motilidade sperm outros motility

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