AvaliaÃÃo do Ãster de acridina como marcador conjugado à lectina em histoquÃmica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Neoplasic cell transformations present differences in the composition and distribution of oligosaccharides in cell surface glicoproteins and glicolipids. Lectins have been used as auxiliary tools in histopathologic diagnosis to mammary, uterus and brain tissues, for example. Chemiluminescence is a technique with low detection threshold and large dynamic spectrum. Acridinium ester (AE) conjugated to antibodies has been employed in chemiluminescent systems to immunodiagnosis. The present work aimed to conjugate AE to Concanavalin A (Con A) and use it as chemiluminescent histochemistry tool. Biopsies of normal and transformed, infiltrating duct carcinoma (IDC), mammary tissues were used. Photon emission, observed during the breakage of the chemical bound between Con A and AE, was quantified, expressed in relative light units (RLU) and correlated to the labeling of the normal and transformed tissues. Results demonstrated that there is RLU proportionality with the labeling. RLU to normal tissue (2,565x103 Â 0,247x103) were lower than to IDC (1.283,920x103 Â 220,621x103). Con A-AE conjugation efficiency, differential staining of normal and IDC tissues, and quantification of results contribute to decrease the subjectivity in routine histopathologic diagnoses and indicate that acrydinum ester can join other lectin marker to be used in histochemistry

ASSUNTO(S)

transformaÃÃes neoplÃsicas bioquimica lectina histoquÃmica

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