Auxins induce clustering of the auxin-binding protein at the surface of maize coleoptile protoplasts.
AUTOR(ES)
Diekmann, W
RESUMO
The predominant localization of the major auxin-binding protein (ABP1) of maize is within the lumen of the endoplasmic reticulum. Nevertheless, all the electrophysiological evidence supporting a receptor role for ABP1 implies that a functionally important fraction of the protein must reside at the outer face of the plasma membrane. Using methods of protoplast preparation designed to minimize proteolysis, we report the detection of ABP at the surface of maize coleoptile protoplasts by the technique of silver-enhanced immunogold viewed by epipolarization microscopy. We also show that ABP clusters following auxin treatment and that this response is temperature-dependent and auxin-specific.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=42179Documentos Relacionados
- Specificity of Auxin-binding Sites on Maize Coleoptile Membranes as Possible Receptor Sites for Auxin Action 1
- Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity.
- Authentic processing and targeting of active maize auxin-binding protein in the baculovirus expression system.
- Crystal structure of auxin-binding protein 1 in complex with auxin
- An auxin-binding protein is localized to the plasma membrane of maize coleoptile cells: identification by photoaffinity labeling and purification of a 23-kda polypeptide.
