Automation of microbial enumeration: development of a disposable hydrophobic grid-membrane filter unit.
AUTOR(ES)
Tsuji, K
RESUMO
A disposable filter unit containing a hydrophobic grid-membrane filter (HGMF) was developed. The unit is liquid tight to serve as a specimen transport container and, by removal of the funnel extender (175- or 300-ml capacity), the unit becomes less than the height of two stacked petri plates to save space during in situ incubation. The polyethylene mesh which supports the HGMF facilitates rinse removal of any substance(s) that would interfere with microbial growth. The correlations between a pour plate, a conventional square HGMF, and a disposable filter unit on microbial enumeration were examined. Characteristics (e.g., clumping, spreading, etc.) of some microorganisms limit the linear counting range to less than 1,000 CFU per filter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239127Documentos Relacionados
- Hydrophobic Grid-Membrane Filters: New Approach to Microbiological Enumeration
- Automated food microbiology: potential for the hydrophobic grid-membrane filter.
- Enumeration of High Numbers of Bacteria Using Hydrophobic Grid-Membrane Filters
- Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe.
- Rapid hydrophobic grid membrane filter-enzyme-labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods.