Attachment of Chloroplast Polysomes to Thylakoid Membranes in Chlamydomonas reinhardtii
AUTOR(ES)
Chua, Nam-Hai
RESUMO
Treatment of synchronous cultures of Chlamydomonas reinhardtii with chloramphenicol at 4 hr after the beginning of the light phase led to a preferential loss of 70S ribosomes from the 17,000 × gmax supernate. The “lost” 70S ribosomes were found associated with a thylakoid membrane fraction prepared from the 17,000 × gmax pellet. Electron microscopic examinations of this fraction revealed that the 70S ribosomes were bound to the unstacked regions of the thylakoid membranes as polygonal penta- and hexamers. These bound ribosomes were only released by treatment with 500 mM KCl and puromycin, suggesting that both ionic interactions and nascent peptide chains were involved in the ribosome-membrane attachment. Since growth of the thylakoid membranes occurs in the light, it is suggested that bound chloroplast ribosomes function in the synthesis of thylakoid membrane proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433541Documentos Relacionados
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