Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment.
AUTOR(ES)
Karrasch, S
RESUMO
The atomic force microscope has the potential to monitor structural changes of a biological system in its native environment. To correlate them with the biological function at a molecular level, high lateral and vertical resolution are required. Here we demonstrate that the atomic force microscope is capable of imaging the surface of the hexagonally packed intermediate layer of Deinococcus radiodurans in buffer solution with a lateral resolution of 1 nm and a vertical resolution of 0.1 nm. On average, these topographs differ from those determined by electron microscopy by <0.5 nm.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=521406Documentos Relacionados
- Structural Information, Resolution, and Noise in High-Resolution Atomic Force Microscopy Topographs
- Contact-Mode High-Resolution High-Speed Atomic Force Microscopy Movies of the Purple Membrane
- Carbon nanotube atomic force microscopy tips: Direct growth by chemical vapor deposition and application to high-resolution imaging
- Automatic extraction of road seeds from high-resolution aerial images
- Water polygons in high-resolution protein crystal structures