Atividade hemaglutinante e larvicida (Aedes aegypti) na Ãgua tratada com sementes de Moringa oleifera

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Moringa oleifera seeds have been frequently used as coagulant for water treatment in Brazilian Northeast, where a high incidence of dengue fever. Lectins constitute a heterogeneous protein group capable of recognizing and reversibly binding to carbohydrates and glycoproteins. Protease inhibitors disrupt enzymatic action by formation of stable complexes with trypsin and/or chymotrypsin. Lectins and trypsin inhibitors with insecticidal activity have been described. The aim of this work was to evaluate hemagglutinating (HA), trypsin inhibitor and larvicidal activities, in the water treated with M. Oleifera seeds. Distilled water was treated with 1, 3, 6 or 15 seeds of M. oleifera (MoW). Lectin assay used rabbit erythrocytes. MoW was also evaluated by trypsin inhibitory activity using N-α-benzoyl-DL-arginyl-ρ-nitroanilide (BAPNA). Larvicidal bioassay was performed using larvae of dengue vector Aedes aegypti. Development, mortality and morphological aspects of larvae were analyzed. MoW contains HA and was not able to inhibit trypsin. Reduction of larvae development was observed after 24, 48 and 72 h of incubation with MoW. Fourth instar larvae (L4) were only detected in control, MoW1 and MoW3. Significant (p<0. 0001) larval mortality was detected in the most active lectin preparation, MoW15. Morphological changes in L4 incubated with MoW1 were demonstrated at light microscope level. The results obtained suggest the evaluation of M. oleifera seeds for the control of dengue vector. The presence of HA in MoW preparations active on A. aegypti larvae can be indicative of lectin involvement in MoW larvicidal effect

ASSUNTO(S)

larvicidal activity aedes aegypti moringa oleifera bioquimica lectin moringa oleifera larvicidal activity aedes aegypti lectin

ACESSO AO ARTIGO

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