Atividade física e suplementação nutricional de leucina associadas ao crescimento tumoral : estudo do perfil hormonal de ratos implantados com carcinossarcomia de Walker 256 / Physical activity and nutritional supplementation with leucine associated with tumor growth : study of hormonal profile in Walker 256

AUTOR(ES)

Aline Tatiane Toneto Inocêncio

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

24/02/2012

RESUMO

The cancer-cachexia is characterized by the development of anorexia, asthenia, weight loss, early satiety, anaemia and especially changes in the metabolism of carbohydrate, fat and protein in these cancer patients. The amino acid leucine acts promoting cell signalling by inhibiting the protein catabolism and stimulating the protein synthesis in skeletal muscle, especially in experimental animals. The exercise provides metabolic changes mainly in protein turnover. Thus studies that concern cancer-cachexia, physical exercise and nutritional supplementation can come up with best ways to support cancer treatment. In this work we evaluated the effects of physical training associated with leucine-rich diet on the metabolic processes and hormonal changes during Walker tumour growth in rats. Wistar rats (45 days-old) were submitted to physical training (swim section for 45 minutes daily, five days a week for 6 weeks). After 6 weeks of training, the rats received subcutaneous implant of Walker carcinoma cells. After 21 days of tumour growth, animals were sacrificed and collected blood, tissues and organs (adrenal, liver, heart and muscle). Cell primary culture of adrenal gland analysed the catecholamine synthesis and cytokines release in the culture medium, which was altered under tumour growth effects and modulated by the effects of leucine-rich diet associated with exercise physical. Tumour growth promoted metabolic, biochemical and hormonal changes in host such as weight loss and increased catabolic hormones release (ACTH and glucagon). The exercise decreased IL-6 and IFN-? content in culture medium and increased IL-4 and IL-10 serum content. Exercise and leucine supplementation provided maintenance of total serum protein and albumin, probably due to leucine which is known as cell signalling and stimulates protein metabolism. In addition, tumour-bearing-exercised animals (WLE) maintained serum protein and albumin, and also the ACTH and glucagon levels, and reduced serum catecholamine levels, in relation to LE group. The biochemical analyses, hormonal and cell culture have shown that exercise and leucine supplementation could improve the cachectic state in experimental animals.

ASSUNTO(S)

exercícios físicos cancer leucina hormônios tumor walker 256 caquexia physical exercises cancer leucine hormones walker 256 tumor cachexia

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