Atividade anticancer de extratos e principios ativos obtidos de Virola sebifera (Myristicaceae) / Anticancer activity of extracts and active principles from Virola sebifera (Myristicaceae)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Cytotoxicity screening models provide important preliminary data to help select plant extracts with potential antitumor properties for future work. As part of a screen program searching for Brazilian medicinal plants and natural products with anticancer properties, the present investigation reports the anticancer activity the Virola sebifera (Myristicaceae), an endemic tree from the Brazilian Cerrado. The overall aim of this work was the evaluation of in vitro and in vivo properties of samples from V. sebifera, there for the isolation and identification of the active compounds. The first study describes the antiproliferative properties against human cell lines of two lignans and two polyketides isolated from leaves of V. sebifera. Herein we report the isolation and structure elucidation of a novel polyketide 3,5-dihydro-2-(r-oxo-3J-hexadecenyl)-2-cyclohexen-l-one with high antiproliferative activity against 8 human cancer cell lines. In the second study, we purified the active fraction (FR4) from the crude dichloromethanic extract with in vitro activity (IC5ri=5.19 - 10.57 pg/mL - SRB assay) against the same cell lines. In addition, the hollow fiber assay was used to study the effect in vivo of the active fraction (FR4). Using this model, we have established growth conditions for MCF-7, NCI-ADR and OVCAR03 cells implanted at the intraperitoneal (i.p.) and subcutaneous (s.c.) compartments of immunocompetent mice. Then, the animals were treated with FR4 (500 mg/kg). doxorubicin (6mg/kg) or vehicle only intraperitoneally. After 14 days the fibers were retrieved, followed by determination of living cell density by MTT staining. FR4 showed significant effect against breast (MCF-7) and ovarian (OVCAR03) tumor cells. The anticancer properties of Virola sebifera in vitro and in vivo was confirmed in this study. These findings may guide future studies in other models and detailed studies on the mechanisms of action.

ASSUNTO(S)

lignanas plants plantas medicinais celulas - cultura lignans medicinal alcaloides cell culture alkaloids

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