Atividade anti-trichomonas vaginalis de moléculas produzidas por basidiomicetos

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

2011

RESUMO

The flagellated protozoan, Trichomonas vaginalis, causes trichomonosis, the most common non-viral sexual transmitted disease (STD) in the world. Metronidazole and tinidazole are two drugs of choice recommended by Food and Drug Administration (FDA, USA) for treatment of human trichomonosis. However, clinical or laboratory-generated drug-resistant isolates of T. vaginalis and common adverse reactions have been reported. It is known that fungi produce bioactive molecules and they can be a potential source of new antiparasitic molecules. Thus, in order to improve the current chemotherapy of T. vaginalis infection, the search for natural bioactive compounds in basidiomycetes can be an interesting approach to discover alternative drugs. The aim of this study was to investigate the presence, to purify and to identify substances in cultivation of basidiomycetes¿ mycelia, which have anti-Trichomonas activity. Mycelia of basidiomycete Amauroderma camerarium, family Ganodermataceae, and Gymnopilus pampeanus, family Cortinariceae, were transferred to flasks containing malt extract broth (MEB) medium with variation of the nitrogen source and time of growth. The preparation with higher anti-T. vaginalis activity (MEB + KNO3 28 days of growth) for A. camerarium basidiomycete was chosen for purification and characterization assays, that indicated that the bioactive compound is a protein-like molecule. A protein without high homology with any other protein was found and was named amaurocine. Amaurocine presents activity against clinical isolates of T. vaginalis ¿ TV LACH1 and TV LACM2 ¿ (MIC=62.4 ¿g/mL), one of these a metronidazole-resistant isolate, and it presents activity against the T. vaginalis ATCC 30236 isolate (MIC=31.2 ¿g/mL). Amaurocine also presents proinflamatory activity. Since it was able to enhance nitric oxide release from neutrophils, which is an important host defense mechanism during the parasitic infection. For G. pampeanus, the most active extracts were produced in MEB + KNO3 28 days of growth and MEB + KNO3 21 days of growth, but further steps of purification are necessary. These results are in agreement with the high potential of biocompounds production of basidiomycetes that has been reported in the literature for decades.

ASSUNTO(S)

trichomonas vaginalis basidiomicetos amauroderma camerarium cogumelos gymnopilus pampeanus antiparasitic compounds atividade antiparasitária bioactive mushroom

ACESSO AO ARTIGO

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