Association of eosinophil cationic protein gene polymorphism with tumor-associated tissue eosinophilia in oral squamous cell carcinomas / Associação do polimorfismo do gene da proteína catiônica eosinofílica com a eosinofilia tecidual associada aos tumores em carcinomas espinocelulares de boca

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Eosinophil cationic protein (ECP), found in secretory granules of human eosinophils, presents cytotoxic activity, particularly against cancer cells. The specific functional role of eosinophils in solid malignant tumors remains unclear. The aim of this study was to investigate the prevalence of the ECP-gene polymorphism 434(G>C) in oral squamous cell carcinoma (OSCC) patients and its association with tumor-associated tissue eosinophilia (TATE), as well as demographic, clinical and microscopic variables. The 434 genotypes in the ECP-gene of 165 healthy individuals and 157 OSCC patients, submitted to surgical treatment at the Hospital A.C. Camargo from 1984 to 2002, were detected by cleavage of the amplified DNA sequence with restriction enzyme PstI and analyses of the cleaved product by agarose gel electrophoresis. TATE, in OSCC, was obtained by morphometric analysis. Chisquare test or Fishers exact test was used to analyze the association among ECP-gene polymorphism 434(G>C), TATE, demographic, clinical and microscopic variables. Diseasefree survival and overall survival were calculated by the Kaplan-Meier product-limit actuarial method and the comparison of the survival curves were performed using log rank test. Most of healthy individuals and OSCC patients showed the genotype 434GC. There was no statistical association among 434 genotypes, TATE intensity and demographic, clinical or microscopic variables of OSCC patients. Higher frequency of bilateral neck dissection, local recurrence, vascular embolization, involved resection margins and postoperative radiotherapy was detected in OSCC patients with intense TATE and 434GC/CC genotypes. No statistically significant differences on survival rates were found among 434 genotypes. In conclusion, these results suggest a tendency of worse clinical outcome in OSCC patients with intense TATE and 434GC/CC genotypes, probably due an ECP genetic variant with altered cytotoxic activity.

ASSUNTO(S)

eosinófilos ecp prognosis carcinoma espinocelular eosinophils gene polymorphism polimorfismo genético ecp squamous cell carcinoma prognóstico

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