Association of cyclic GMP with gene expression of polytene chromosomes of Drosophila melanogaster.
AUTOR(ES)
Spruill, W A
RESUMO
The distribution of cyclic nucleotides on polytene chromosomes isolated from Drosophilia melanogaster salivary glands was examined by using an indirect immunofluorescent technique. With a fixative that minimized the loss of chromosomal proteins, cyclic GMP, but not cyclic AMP, was observed distributed along the chromosomes. The subchromosomal distribution of cyclic GMP correlated with genetically active sites on the chromosomes. After heat-shock treatments, the intensity of cyclic GMP fluorescence was markedly enhanced at specific loci on the chromosomes, with locus 93D as the most intensely fluorescent. Autoradiographic analysis with [3H]uridine revealed that 93D was the most transcriptionally active locus within a particular nucleus. These observations suggest that cyclic GMP may participate in processes associated with transcription on polytene chromosomes. The involvement of cyclic GMP in nuclear events associated with gene expression is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411496Documentos Relacionados
- Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster.
- Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster.
- Puffing activities and binding of ecdysteroid to polytene chromosomes of Drosophila melanogaster.
- Direct interaction of the Polycomb protein with Antennapedia regulatory sequences in polytene chromosomes of Drosophila melanogaster.
- Differential distribution of RNA polymerase B and nonhistone chromosomal proteins in polytene chromosomes of Drosophila melanogaster.
