Assessment of two methods for rapid intrapartum detection of vaginal group B streptococcal colonisation.
AUTOR(ES)
Simpson, A J
RESUMO
AIMS--To compare two methods for the rapid detection of intrapartum vaginal carriage of group B streptococci (Streptococcus agalactiae) with standard culture techniques and to establish their suitability for routine use. METHODS--Vaginal swabs from 266 patients in labour were incubated in glucose broth in an anaerobic atmosphere for four to six hours. The Wellcogen Strep B latex particle agglutination test kit was subsequently used for antigen detection. In the second part of the study swabs from 117 women were assessed for the presence of group B streptococci using the ICON STREP B immuno-concentration assay (Hybritech). Both methods were compared with standard semiquantitative culture on Columbia horse blood agar and Islam's medium. RESULTS--In the first study vaginal carriage of group B streptococci was shown in 38 of 266 (14.3%) patients by culture. Latex particle agglutination with the Wellcogen kit detected 30 of these positive results (sensitivity 78.9%, specificity 100%). In those patients with moderate to heavy colonisation (> 10(4) colony forming units per millilitre) antigen was detected in all (26/26) culture positive patients (sensitivity 100%, specificity 100%). In the second study 16 (13.7%) patients were culture positive. The ICON test detected 11 positive results (sensitivity 68.8%, specificity 100%) and for heavy colonisation (10(5) cfu/ml) detected nine of nine cases (sensitivity 100%, specificity 100%). The ICON test took 10 to 15 minutes to perform. CONCLUSION--These tests are potentially useful for the rapid detection of group B streptococci vaginal colonisation in labour, particularly heavy colonisation. Both tests are insufficiently sensitive to replace standard culture methods.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=502152Documentos Relacionados
- Comparison of two antigen assays for rapid intrapartum detection of vaginal group B streptococcal colonization.
- Rapid diagnosis of intrapartum group B streptococcal carriage by fluorescent in situ hybridisation
- Evaluation of four methods for detection of group B streptococcal colonization.
- Rapid Detection of Group B Streptococci Directly from Vaginal Swabs
- Rapid detection of group B streptococci directly from vaginal swabs.