Assessment of the relationship between signal intensities and transcript concentration for Affymetrix GeneChip® arrays

AUTOR(ES)

Chudin, Eugene

FONTE

BioMed Central

RESUMO

Affymetrix microarrays have been shown to give a signal proportional to actual transcript concentration, to distinguish concentration levels within a factor of 2, and to detect transcript frequencies as low as 1 in 2,000,000. We assessed these representations through a series of 'spike-in' hybridizations involving four prokaryotic transcripts in the absence and presence of fixed eukaryotic background. A linear relationship between transcript abundance and signal was consistently observed between 1 pM and 10 pM transcripts. Landmark studies outlining an observed linear relationship between signal and transcript concentration may not extend to commercially available arrays under routine operating conditions.

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