Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays

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BioMed Central

RESUMO

Long oligonucleotide microarrays are potentially more cost- and management efficient than cDNA microarrays. Unmodified sense and antisense 70-mer oligonucleotides were synthesized and compared with PCR-amplified cDNA clones corresponding to the same genes. The correlation coefficient between oligonucleotide and cDNA probes for identifying differentially expressed genes was 0.80.

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