Aspects of staining, life cycle, parasitism by Pasteuria penetrans and their relationships to body energy reserve of second stage juveniles of Meloidogyne spp. / âAspectos da coloraÃÃo, ciclo de vida, parasitismo por Pasteuria penetrans e suas relaÃÃes com a reserva energÃtica de juvenis do segundo estÃdio de Meloidogyne spp.â

AUTOR(ES)

Fernando da Silva Rocha

DATA DE PUBLICAÇÃO

2007

RESUMO

Several aspects related to nematode staining, migration, development, reproduction, infection by P. penetrans and their relationships to the body energy reserve of second stage juveniles (J2) of Meloidogyne spp. were studied. A new procedure for nematode staining was developed using bordeaux and red bordeaux stains found in the powdered juices Q.refresco flavor strawberry and grape and Ki.Gostoso flavor blackcurrant. The color intensity produced by these stains was similar to the obtained with Phoxine B and acid fuchsin. This method is currently being used in the routine of UFLAâS Nematology Laboratory. Field studies showed that the amount of body lipids varied in M. exigua J2 during the year, with the lowest concentrations found in August and the highest from November to February. When J2 of this nematode were stored in water for three days, there was a decrease in the lipid content followed by a reduction on infectivity and reproduction in the cultivars Nemadoro and Kada of tomato. Meloidogyne incognita stored in sand also showed reduction on J2 lipid content from the fifth day of storage onward. The migration was reduced according to the increase of the distance between the inoculum source and the soybean roots. The storage in water of M. incognita J2 with P. penetrans endospores adhered to the cuticle caused less reduction in J2 lipid content when compared to J2 without endospores. The J2 with endospores also had a reduction on parasitism and on the number of endospores produced per female, following the increase of the storage period. The dark area observed along the body of M. incognita J2 in the absence of stains correlated to the area stainned red by âOil Red Oâ, and to the infectivity and reproduction of M. incognita. The neutral lipids also correlated with lipid index, lipid weight and to infectivity and reproduction of M. exigua. Meloidogyne incognita and M. exigua J2 stored for six days lost more than 50% of the neutral lipid content. This level of lipid content and time of storage represent the threshold for tomato infectivity, whereas for reproduction the threshold was three days of storage. The results of this study will contribute to the better understanding of the role of body energy reserve in J2 of Meloidogyne spp. in their life cycle on roots of host plants.

ASSUNTO(S)

fitopatologia parasitism method of staining mÃtodos de coloraÃÃo meloidogyne incognita lipÃdios neutros meloidogyne exigua neutral lipid reproduction parasitismo pasteuria penetrans meloidogyne exigua meloidogyne incognita pasteuria penetrans

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