aspects humoral immune response and cells of cattle naturally infected with Mycobacterium bovis and EVALUATION OF VACCINE FOR TUBERCULOSIS IN protein subunits in mice / aspects humoral immune response and cells of cattle naturally infected with Mycobacterium bovis and EVALUATION OF VACCINE FOR TUBERCULOSIS IN protein subunits in mice / ASPECTOS DA RESPOSTA IMUNE HUMORAL E CELULAR DE BOVINOS NATURALMENTE INFECTADOS COM MYCOBACTERIUM BOVIS E AVALIAÇÃO DE VACINA DE SUBUNIDADE PROTÉICA PARA TUBERCULOSE EM CAMUNDONGOS / ASPECTOS DA RESPOSTA IMUNE HUMORAL E CELULAR DE BOVINOS NATURALMENTE INFECTADOS COM MYCOBACTERIUM BOVIS E AVALIAÇÃO DE VACINA DE SUBUNIDADE PROTÉICA PARA TUBERCULOSE EM CAMUNDONGOS

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Several aspects of the bovine tuberculosis were analyzed in this study. The immunogenicity of recombinant MPT-51 (rMPT-51), Ag-85 and M. bovis-BCG were characterized in an immunosorbent assay, where 208 serum samples from positive intradermal tuberculin test (ITT) animals and 54 serum samples from ITT negative animals where analyzed. M. bovis-BCG and Ag-85 were strongly recognized by antibodies from naturally infected cattle. Additionally, the clinical status of the animals were correlated with the ITT positivity, with the specific production of IL-4 by TCD4 and TCD8 positive lymphocytes, and with nitric oxide (NO) production by macrophages from naturally tuberculosis infected bovine peripheral blood. ITT positive animals showed TCD4+IL4+ cells specific to M. bovis-BCG extract. High background levels of TCD8+IL-4+ lymphocytes were observed in ITT positive animals independent of the stimuli. When cell cultures where stimulated with M. bovis-BCG protein extract, there was no observed difference in NO production between the groups. Naturally tuberculosis infected bovine presented TCD4+IL4+ cells specific for M. bovis-BCG and a preserved NO production. Finnally, the immunogenicity of rMPT-51 use as a proteic sub-unit vaccine was evaluated in BALB/c mice, with two different adjuvants, incomplete Freund and CpG DNA. For this, mice were immunized and challenged with M. tuberculosis. Immunization with rMPT-51 antigen and either adjuvant induced, in the lungs, a migration increase of TCD5+IFN+ cells specific for rMPT-51, when compared to controls (P<0.05). rMPT-51 plus CpG DNA presented a better performance among the different vaccination schemes tested, in part due to the ability of stiulate TCD5+IFN+ cells and hampering the bacterial load, thus preserving the functional integrity of challenged mice lungs

ASSUNTO(S)

antígenos recombinantes, bcg, bovino, diagnóstico, tuberculose, vacina clinica e cirurgia animal recombinant antigens, bcg, bovine, diagnostic, tuberculosis, vaccine

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