Aspectos TaxonÃmicos e FilogenÃticos em Fungos MicorrÃzicos arbusculares (Glomeromycota) / Aspectos TaxonÃmicos e FilogenÃticos em Fungos MicorrÃzicos Arbusculares (Glomeromycota)




The arbuscular mycorrhizal fungi (AMF) form symbiotic association with most plants. From data of SSU rDNA, these fungi were recently grouped in a new phylum Glomeromycota); however much is still unclear in the classification of the group. Thus, the objectives of this work were: (a) to determine the evolutive relations in Glomeromycota using morphological and molecular data, and constructing phylogenetic trees; (b) suggest new hypothesis regarding the phylogenetic relations of the group, as well as the utilization of other genes, besides the rDNA, in the cladistic evaluation; (c) contribute for the diagnostic of species in Gigaspora; (d) to build keys for identification of species in Scutellospora. Partial sequences of LSU rDNA and morphological data were used in the phylogenesis of Glomeromycota. The trees obtained indicated that: the phylum is monophyletic, Acaulosporaceae and Gigasporaceae are united in the same clade, and Glomeraceae is poliphyletic, as previously described. However, although the results support most of the data shown by other researchers, other species should be evaluated for determination of groups still not understood in the phylum. Sequences of possible mitochondrial genes for phylogenetic evaluation of Gigaspora were also analyzed and at least one of them was phylogenetic-informative. In order to help the identification of Scutellospora species, dichotomic keys using morphological data were constructed. A species-specific primer was also designed for G. albida, because this species has been morphologically confused with others (G. margarita and G. rosea) of the same genera


primer taxonomia rdna glomeromycota taxonomy micorriza arbuscular rdna filogenia glomeromycota micologia primer phylogeny arbuscular mycorrhiza

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