Aromatic Compound-Dependent Brucella suis Is Attenuated in Both Cultured Cells and Mouse Models

AUTOR(ES)

Foulongne, Vincent

FONTE

American Society for Microbiology

RESUMO

The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was highly attenuated in tissue culture (THP1 macrophages and HeLa cells) and murine virulence models.

Documentos Relacionados

• Immunization against anthrax with aromatic compound-dependent (Aro-) mutants of Bacillus anthracis and with recombinant strains of Bacillus subtilis that produce anthrax protective antigen.
• Interleukin-12 Is Required for Control of the Growth of Attenuated Aromatic-Compound-Dependent Salmonellae in BALB/c Mice: Role of Gamma Interferon and Macrophage Activation
• Impairment of Intramacrophagic Brucella suis Multiplication by Human Natural Killer Cells through a Contact-Dependent Mechanism
• The Brucella suis virB operon is induced intracellularly in macrophages
• Proliferation is necessary for both repair and mutation in transgenic mouse cells