Approaches for in silico finishing of microbial genome sequences
AUTOR(ES)
Kremer, Frederico Schmitt, McBride, Alan John Alexander, Pinto, Luciano da Silva
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
2017-09
RESUMO
Abstract The introduction of next-generation sequencing (NGS) had a significant effect on the availability of genomic information, leading to an increase in the number of sequenced genomes from a large spectrum of organisms. Unfortunately, due to the limitations implied by the short-read sequencing platforms, most of these newly sequenced genomes remained as “drafts”, incomplete representations of the whole genetic content. The previous genome sequencing studies indicated that finishing a genome sequenced by NGS, even bacteria, may require additional sequencing to fill the gaps, making the entire process very expensive. As such, several in silico approaches have been developed to optimize the genome assemblies and facilitate the finishing process. The present review aims to explore some free (open source, in many cases) tools that are available to facilitate genome finishing.
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