Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni

AUTOR(ES)

Oliveira, Edward José de, Kanamura, Hermínia Yohko, Takei, Kioko, Hirata, Rosario Dominguez Crespo, Nguyen, Nga Yen, Hirata, Mário Hiroyuki

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2006-10

RESUMO

The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.

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