Application of Next-generation Sequencing in Clinical Molecular Diagnostics

AUTOR(ES)

Seifi, Morteza, Ghasemi, Asghar, Raeisi, Sina, Heidarzadeh, Siamak

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

18/05/2017

RESUMO

ABSTRACT Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy. Over the past years, many academic companies and institutions have continued technological advances to expand NGS applications from research to the clinic. In this review, the performance and technical features of current NGS platforms were described. Furthermore, advances in the applying of NGS technologies towards the progress of clinical molecular diagnostics were emphasized. General advantages and disadvantages of each sequencing system are summarized and compared to guide the selection of NGS platforms for specific research aims.

Documentos Relacionados

• Next-generation Sequencing-based genomic profiling: Fostering innovation in cancer care?
• The Role of Next-Generation Sequencing in the Diagnosis of Lysosomal Storage Disorders
• Glycogen Storage Diseases: Next-Generation Medicine
• Production of the next-generation library virtual tour
• Hereditary cancer risk assessment: insights and perspectives for the Next-Generation Sequencing era