Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing Escherichia coli in Bloody Stools: Identification of Serogroups O26 and O111

AUTOR(ES)

Louie, M.

FONTE

American Society for Microbiology

RESUMO

Primers were designed to amplify sequences of verocytotoxin genes and eaeA genes of Escherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detected E. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producing E. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.

Documentos Relacionados

• Occurrence of Verocytotoxin-Producing Escherichia coli O157 on Dutch Dairy Farms
• Infection by verocytotoxin-producing Escherichia coli.
• Detection and Characterization of Verocytotoxin-Producing Escherichia coli by Automated 5′ Nuclease PCR Assay
• Isolation and Characterization of Verocytotoxin-Producing Escherichia coli O157 Strains from Dutch Cattle and Sheep
• Signal transduction responses following adhesion of verocytotoxin-producing Escherichia coli.