Aperfeiçoamento da fermentação de sacarose através da modificação da expressão dos genes SUC2 e AGT1 em linhagens diploides de Saccharomyces cerevisiae. / Improvement of sucrose fermentation by modifying the expression of SUC2 and AGT1 genes in diploid Saccharomyces cerevisiae strains.

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

20/03/2012

RESUMO

Currently, the S. cerevisiae strains used in industrial production of fuel alcohol in Brazil are diploid yeasts that of metabolize sucrose through its extracellular hydrolysis by the periplasmic invertase, followed by the transport of the formed glucose and fructose into the cells, and further metabolism through glycolysis. In this study, utilizing genetic engineering techniques and further crosses, diploid strains of S. cerevisiae were developed that are able to consume this sugar through its direct uptake by the transporter encoded by the AGT1 gene, and intracellular hydrolysis by the cytoplasmic invertase encoded by a modified version of the SUC2 gene. These modifications allowed achieving a 10% higher yield in the production of ethanol, besides increasing the sucrose consumption rate and decreasing the amount of residual sugars at the end of the process. These results open new horizons to turn ethanol production in Brazil more efficient.

ASSUNTO(S)

saccharomyces saccharomyces alcoholic fermentation expressão gênica fermentação alcoólica gene expression gene regulation regulação gênica sacarose sucrose

ACESSO AO ARTIGO

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