Antiviral Effect of Interferon Covalently Bound to Sepharose
AUTOR(ES)
Ankel, H.
RESUMO
Interferon, covalently bound to Sepharose 4B activated by cyanogen bromide, induces the antiviral state in sensitive cells. The antiviral effect is neutralized by antiserum specific to interferon and is recovered thereafter when the antibody is detached from the interferon by treatment at low pH. Binding interferon to Sepharose increases the stability of the molecule. It is likely that the interferon molecule acts on the cell receptor without being detached from the beads. However, the data do not exclude the possibility of a small loss of interferon, or fragments of it, after contact with the cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433735Documentos Relacionados
- Inhibition of the Bacillus subtilis Membrane-Bound d-Alanine Carboxypeptidase by 6-Aminopenicillanic Acid Covalently Coupled to Sepharose
- Affinity isolation of cultured tumor cells by means of drugs and hormones covalently bound to glass and Sepharose beads.
- Biologically Active Catecholamines Covalently Bound to Glass Beads
- Isolation of rabbit reticulocyte initiation factors by means of heparin bound to sepharose.
- Purification of a Specific tRNA by Sepharose-Bound Enzyme*