Antigenicity of converting phages obtained from Clostridium botulinum types C and D.
AUTOR(ES)
Oguma, K
RESUMO
Phage conversion of toxigenicity in Clostridium botulinum types C and D was accomplished by using nontoxigenic strains and phages purified from plaques. Although the morphology of the converting phages seemed to be the same, they were divided into three groups on the basis of their conversion spectrum. The first group consists of phages obtained from toxogenic strains C-Stockholm and C-468. The second group consists of phages from strains D-1873 and C-203. The third group consists of phages from strains D-South African and D-4947. These converting phages were also classified into the same three groups by a neutralization test with specific antiphage sera. Cross-neutralization, however, was observed between phages belonging to group 1 and group 2,by both the neutralization test of converting ability and by a plaque experiment in which the surviving rates of phages were calculated after treatment with each antiphage serum. The antigenic differences among these converting phages should probably comprise one of the reasons for the existence of the specific infection spectrum in C. botulinum types C and D.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=420688Documentos Relacionados
- Characterization of bacteriophage nucleic acids obtained from Clostridium botulinum types C and D.
- Phage conversion to hemagglutinin production in Clostridium botulinum types C and D.
- Use of ganglioside affinity filters to identify toxigenic strains of Clostridium botulinum types C and D.
- Comparative analysis of C3 and botulinal neurotoxin genes and their environment in Clostridium botulinum types C and D.
- Comparison of antigenicity of toxins produced by Clostridium botulinum type C and D strains.