Antidiarrheal effect of extract from the bark of Combretum leprosum in mice
AUTOR(ES)
CAVALCANTI, PAULO M.S., MARTINS, MARIA DO CARMO C., NUNES, PAULO H.M., ALVES FILHO, FRANCISCO C., SILVA, JANYERSON D.P., CAVALCANTI, SUZANA M.G.
FONTE
An. Acad. Bras. Ciênc.
DATA DE PUBLICAÇÃO
17/12/2018
RESUMO
Abstract This study investigated the effects of the ethanolic extract from the bark of Combretum leprosum (ECL) on intestinal transit and castor-oil induced diarrhea in mice. The oral administration of ECL (750 and 1000 mg/kg) slowed intestinal transit (ID50 of 455 mg/kg). The ECL (250-1000 mg/kg) reduced castor-oil induced diarrhea, in a time- and dose-dependent manner (p < 0.05). To determine if antidiarrheal effect of ECL involves α2-adrenergic or opioid receptor activation, the mice were pretreated with antagonists of these receptors, yohimbine or naloxone respectively. None of these drugs inhibited the antidiarrheal effect of ECL. To test if antidiarrheal effect of ECL is due to an antisecretory action, we realized the enteropooling assay on rats. The ECL increased bowel content and did not inhibit intestinal fluid secretion increase induced by misoprostol (100 µg/kg, s.c.). To determine if antimotility effect of ECL is due to a reduction on gastric motility, we realized the organ bath assay in the rat fundus stomach. Isotonic recordings show that the carbachol /KCl - induced contraction was not reversed by the addition of ECL. In conclusion, our results suggest that ECL contains antidiarrheal compounds and these compounds could induce a reduction of intestinal tract motility.
Documentos Relacionados
- Antinociceptive effect on mice of the hydroalcoholic fraction and (-) epicatechin obtained from Combretum leprosum Mart & Eich
- Activity of the Lupane isolated from Combretum leprosum against Leishmania amazonensis promastigotes
- Natural and Semisynthetic Triterpenes from Combretum leprosum Mart. with Antiplasmodial Activity
- Phytochemical study and antinociceptive effect of the hexanic extract of leaves from Combretum duarteanum and friedelin, a triterpene isolated from the hexanic extract, in orofacial nociceptive protocols
- Anti-inflammatory effect of the ethanolic extract from Bowdichia virgilioides H.B.K stem bark