Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

Rodrigues, José Ariévilo Gurgel, Vanderlei, Edfranck de Sousa Oliveira, Bessa, Érika Façanha, Magalhães, Francisco de Araújo, Paula, Regina Célia Monteiro de, Lima, Vilma, Benevides, Norma Maria Barros

Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

AUTOR(ES)

Rodrigues, José Ariévilo Gurgel, Vanderlei, Edfranck de Sousa Oliveira, Bessa, Érika Façanha, Magalhães, Francisco de Araújo, Paula, Regina Célia Monteiro de, Lima, Vilma, Benevides, Norma Maria Barros

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2011-08

RESUMO

The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg-1), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.